Dengue : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Padmanabhan, Radhakrishnan. (Editor ), Vasudevan, Subhash G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1138
Materias:
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024 7 |a 10.1007/978-1-4939-0348-1  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Dengue :  |b Methods and Protocols /  |c edited by Radhakrishnan Padmanabhan, Subhash G. Vasudevan. 
250 |a 1st ed. 2014. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2014. 
300 |a XIII, 403 p. 82 illus., 49 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1138 
505 0 |a Dengue Virus Growth, Purification and Fluorescent Labeling -- Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique -- Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique -- Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy -- Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion -- Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection -- Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging -- Development and Application of Dengue Virus Reverse Genetic Systems -- DENV4 Renilla Luciferase Reporter Replicons -- Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination -- Identification of Dengue-Specific Human Antibody Fragments Using Phage Display -- Next Generation Whole Genome Sequencing of Dengue Virus -- MPGAfold in Dengue Secondary Structure Prediction -- Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE -- Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 -- Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry -- Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore -- Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation -- Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import -- Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening -- Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors -- Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase -- Animal Models in Dengue -- Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model. 
650 0 |a Infectious diseases. 
650 0 |a Virology. 
650 0 |a Laboratory medicine. 
650 1 4 |a Infectious Diseases. 
650 2 4 |a Virology. 
650 2 4 |a Laboratory Medicine. 
700 1 |a Padmanabhan, Radhakrishnan.  |e editor. 
700 1 |a Vasudevan, Subhash G.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks