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161111s2016 gw | s |||| 0|eng d |
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|a 9783319452951
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| 024 |
7 |
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|a 10.1007/978-3-319-45295-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Janetzki, Sylvia.
|e author.
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| 245 |
1 |
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|a Elispot for Rookies (and Experts Too) /
|c by Sylvia Janetzki.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
# |
|a Cham :
|b Springer International Publishing :
|b Imprint: Springer,
|c 2016.
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| 300 |
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|a VIII, 93 p. 39 illus., 32 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Techniques in Life Science and Biomedicine for the Non-Expert,
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| 505 |
0 |
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|a Preface -- Overview -- Historic Overview -- Principles of Elispot -- Modifications -- Simplifications -- Coating Step -- Detection Step -- Adaptations -- Detection of specific antibodies -- Detection of multiple analytes in one well -- Dual color Elispot -- Fluorospot -- Summary -- Important Assay Details -- Reagents and Materials -- Elispot plates -- Antibody choices -- Enzymatic conjugates and substrates -- Fluorescent dyes -- Culture media -- Blocking buffers -- Washing buffers -- Important laboratory supplies -- Pipettes -- Filters -- Other supplies -- Automated Elispot reader -- Sample preparation -- Overview -- Whole blood and PBMC -- Blood draw and anticoagulants -- PBMC isolation -- Time frame between blood draw and PBMC isolation -- Immune cells obtained from tissue -- Freezing of cells -- Thawing of cells -- Cell counting and viability -- Overnight resting -- Isolation of sub-populations of cells -- Expansion of cells -- Planning the experiment -- Controls -- Negative controls -- Positive controls -- External controls -- Plate layout -- Timing -- The Elispot assay -- Coating -- Blocking -- Effector cell preparation -- Stimulant preparation -- Peptides and peptide pools -- Proteins and lysate -- Antigen presenters -- Control stimulants -- Reference samples -- Plating out cells and stimulants -- Incubation -- Removal of cells -- Spot development -- Enumeration of spots -- Data analysis -- Quality control -- Reporting guidelines -- Troubleshooting -- References.
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| 650 |
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0 |
|a Immunology.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Laboratory medicine.
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| 650 |
1 |
4 |
|a Immunology.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Laboratory Medicine.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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