FOXO Transcription Factors : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Link, Wolfgang. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1890
Materias:
Tabla de Contenidos:
  • Introduction to FOXO Biology
  • Characterization of microRNAs Regulating FOXO Expression
  • Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter
  • Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover
  • Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity
  • Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes
  • Characterization of FOXO Acetylation
  • Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays
  • Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes
  • Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters
  • RNAi Mediated Silencing of FoxO Factors
  • Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO
  • High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO
  • Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus
  • Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology
  • Genome-wide Analysis for Identifying FOXO Protein Binding Sites
  • Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors
  • Following Transcriptome to Uncover FOXO Biological Functions
  • Hydra as Model to Determine the Role of FoxO in Longevity
  • Genetic Ablation of FOXO in Mice to Investigate its Physiological Role
  • Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity
  • Analysis of FOXO3a Gene Polymorphism Associated with Asthma. .