Functional Disulphide Bonds : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Hogg, Philip. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1967
Materias:
Tabla de Contenidos:
  • 1. Classification of protein disulfide bonds
  • 2. Assessing the evolutionary conservation of protein disulphide bonds
  • 3. A proteomics workflow for the Identification of labile disulphide bonds at the cell surface
  • 4. Quantification of the redox state of protein disulfide bonds
  • 5. Quantification of the redox state of protein disulfide bonds
  • 6. Studying functional disulfide bonds by computer simulations
  • 7. Dynamic force spectroscopy analysis on the redox states of protein disulfide bonds
  • 8. Assays of thiol isomerase enzymatic activity
  • 9. Functional assays of thiol isomerase ERp5
  • 10. IDENTIFICATION OF PDI SUBSTRATES BY MECHANISM-BASED KINETIC TRAPPING
  • 11. Site-specific proteomic mapping of modified cysteine residues
  • 12. Quantitation of glutathione, glutathione disulfide and protein-glutathione mixed disulfides by high performance liquid chromatography-tandem mass spectrometry
  • 13. Interrogation of Functional Mitochondrial Cysteine Residues by Quantitative Mass Spectrometry
  • 14. Oxidative Protein Folding Using trans-3,4-Dihydroxyselenolane Oxide
  • 15. Methodology for identification of the cysteine-reactive covalent inhibitors
  • 16. In Vivo Measurement of Redox-Regulated TG2 Activity
  • 17. Quantitation of total and free thiol β2-glycoprotein I levels for diagnostic and prognostic purposes in the antiphospholipid syndrome
  • 18. Quantitation of total and free thiol angiotensinogen as a prognostic marker for pre-eclampsia
  • 19. Preparation of a dithiol-reactive probe for PET imaging of cell death
  • 20. Flow Cytometry Assessment of Procoagulant Platelets Using A Dithiol Reactive Probe. .