Gel-Free Proteomics : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Gevaert, Kris. (Editor ), Vandekerckhove, Joël. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 753
Materias:
Tabla de Contenidos:
  • Mass Spectrometry-driven Proteomics: An Introduction
  • Metabolic Labeling of Model Organisms using Heavy Nitrogen (15N)
  • Trypsin-catalyzed Oxygen-18 Labeling for Quantitative Proteomics
  • ICPL Labeling Strategies for Proteome Research
  • Quantitative Proteome Analysis using Isobaric Peptide Termini Labeling (IPTL)
  • Complete Chemical Modification of Amine and Acid Functional Groups of Peptides and Small Proteins
  • Production and Use of Stable-isotope-labeled Proteins for Absolute Quantitative Proteomics
  • Organelle Proteomics
  • Membrane Protein Digestion – Comparison of LPI Hexalane with Traditional Techniques
  • GeLCMS for In-depth Protein Characterization and Advanced Analysis of Proteomes
  • Exploring New Proteome Space: Combining Lys-N Proteolytic Digestion and Strong Cation Exchange (SCX) Separation in Peptide Centric MS-driven Proteomics
  • Quantitation of Newly Synthesized Proteins by Pulse-labeling with Azidohomoalanine
  • Analytical Strategies in Mass Spectrometry Based Phosphoproteomics
  • A Protocol on the Use of Titanium Dioxide Chromatography for Phosphoproteomics
  • Positional Proteomis at the N-terminus as a Means of Proteome Simplification
  • N-terminomics: A High-content Screen for Protease Substrates and their Cleavage Sites
  • Protease Specificity Profiling by Tandem Mass Spectrometry Using Proteome-derived Peptide Libraries
  • Identification of Proteolytic Products and Natural Protein N-termini by Terminal Amine Isotopic Labeling of Substrates (TAILS)
  • Lectins as Tools to Select for Glycosylated Proteins
  • Strong Cation Exchange Chromatography for Analysis of Sialylated Glycopeptides
  • Titanium Dioxide Enrichment of Sialic Acid-containing Glycopeptides
  • Chemical de-O-Glycosylation of Glycoproteins for Applications in LC-based Proteomics
  • Ubiquitination and Degradation of Proteins
  • Bioinformatics Challenges in Mass Spectrometry Driven Proteomics
  • A Case Study on the Comparison of Different Software Tools for Automated Quantification of Peptides.