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151130s2016 xxu| s |||| 0|eng d |
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|a 9781493929542
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| 024 |
7 |
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|a 10.1007/978-1-4939-2954-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Embryonic Stem Cell Protocols /
|c edited by Kursad Turksen.
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| 250 |
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|a 3rd ed. 2016.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XV, 449 p. 84 illus., 49 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1341
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| 505 |
0 |
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|a Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition -- A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells -- Resolving Heterogeneity: Fluorescence Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture -- Acquiring Ground State Pluripotency: Switching Mouse Embryonic Stem Cells from Serum/LIF Medium to 2i/LIF Medium -- Generation of Embryonic Stem Cells in Rabbits -- Gene Transfer into Pluripotent Stem Cells Via Lentiviral Transduction -- Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells -- Expanding the Utility of FUCCI Reporters Using FACS-Based ‘Omics Analysis -- Selection of Antibodies Interfering with Cell-Surface Receptor Signalling Using Embryonic Stem Cell Differentiation -- Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells -- Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells -- Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells -- Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation -- Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers -- Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells -- From Naive to Primed Pluripotency: In Vitro Conversion of Mouse Embryonic Stem Cells in Epiblast Stem Cells -- In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor’s Production -- Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes -- Intracellular Calcium Measurements for Functional Characterization of Neuronal Phenotypes -- In Vitro Differentiation of Pluripotent Stem Cells into Functional β Islets Under 2D and 3D Culture Conditions and In Vivo Preclinical Validation of 3D Islets -- Generation of Corneal Keratocytes from Human Embryonic Stem Cells -- Maintenance, Transgene Delivery, and Pluripotency Measurement of Mouse Embryonic Stem Cells -- Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool -- Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis -- 26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells -- A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells -- Applying Shear Stress to Pluripotent Stem Cells -- Generating Inner Ear Organoids from Mouse Embryonic Stem Cells -- Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells -- Pancreatic Differentiation from Murine Embryonic Stem Cells -- Osteogenic Differentiation from Embryonic Stem Cells -- Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Turksen, Kursad.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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