Epitope Mapping Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Reineke, Ulrich. (Editor), Schutkowski, Mike. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edition:2nd ed. 2009.
Series:Methods in Molecular Biology, 524
Subjects:
Table of Contents:
  • B-cell Epitope Mapping
  • What Is a B-Cell Epitope?
  • Whole Antigen Methods
  • Structural Basis of Antibody-Antigen Interactions
  • Epitope Mapping of Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy
  • A Solid-Phase Mutual Inhibition Assay with Labeled Antigen
  • Epitope Mapping by Surface Plasmon Resonance
  • Proteolytic Fragmentation for Epitope Mapping
  • Epitope Mapping by Proteolysis of Antigen-Antibody Complexes
  • Identifying Residues in Antigenic Determinants by Chemical Modification
  • Epitope Mapping by Differential Chemical Modification of Antigens
  • Peptide Library Approaches
  • Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides
  • Antibody Epitope Mapping Using SPOT™ Peptide Arrays
  • Peptide Microarrays for Profiling of Modification State-Specific Antibodies
  • Epitope Mapping Using Phage Display Peptide Libraries
  • Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries
  • Antibody Specificity Profiling on Functional Protein Microarrays
  • Peptide Microarrays for Determination of Cross-Reactivity
  • Profiling of Antibody Signatures in Biological Fluids.
  • Epitope Mapping Using Randomly Generated Peptide Libraries
  • Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease
  • Microarrayed Allergen Molecules for Diagnostics of Allergy
  • Monitoring B Cell Response to Immunoselected Phage-Displayed Peptides by Microarrays
  • Antigen Expressed from Recombinant DNA
  • Epitope Mapping Using Homolog-Scanning Mutagenesis
  • Epitope Mapping by Region-Specified PCR-Mutagenesis
  • Epitope Mapping Using Phage-Display Random Fragment Libraries
  • B-Cell Epitope Prediction
  • Prediction of Linear B-cell Epitopes
  • T-Cell Epitope Mapping
  • Molecular Recognition of Diverse Ligands by T-Cell Receptors
  • Identification of Human MHC Class I Binding Peptides using the iTOPIA™? Epitope Discovery System
  • T-Cell Epitope Mapping in Mycobacterium tuberculosis Using PepMixes Created by Micro-Scale SPOT™? Synthesis
  • High-Throughput T-Cell Epitope Discovery Through MHC Peptide Exchange
  • T-Cell Epitope Processing (The Epitope Flanking Regions Matter)
  • Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules
  • T-Cell Epitope Mapping
  • Identification and Validation of T-Cell Epitopes Using the IFN-? EliSpot Assay.