Extracellular RNA : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Patel, Tushar. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2018.
Edition:1st ed. 2018.
Series:Methods in Molecular Biology, 1740
Subjects:
Table of Contents:
  • Extracellular RNAs - A New Awareness of Old Perspectives
  • Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
  • Extracellular RNA Isolation from Cell Culture Supernatant
  • Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture
  • Isolation of Extracellular RNA from Serum/Plasma
  • Isolation of Extracellular RNA from Bile
  • Cushioned-Density Gradient Ultracentrifugation (C-DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes
  • Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids
  • Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography
  • Detection and Analysis of Non-vesicular Extracellular RNA
  • Isolation of Plasma Lipoproteins as a Source of Extracellular RNA
  • Droplet Digital PCR for Quantitation of Extracellular RNA
  • Preparation of Small RNA NGS Libraries from Biofluids
  • Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression using NanoString
  • Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
  • Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs. .