DNA Repair Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Henderson, Daryl S. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2006.
Edición:1st ed. 2006.
Colección:Methods in Molecular Biology, 314
Materias:
Tabla de Contenidos:
  • Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating
  • Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes
  • Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities
  • Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation
  • Inhibition of DNA Synthesis by Ionizing Radiation
  • Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication
  • Cytometric Assessment of Histone H2AX Phosphorylation
  • Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication)
  • In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
  • Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR
  • Plasmid-Based Assays for DNA End-Joining In Vitro
  • Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants
  • Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage
  • Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells
  • Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR
  • Immunochemical Detection of UV-Induced DNA Damage and Repair
  • A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts
  • Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
  • DNA Damage Quantitation by Alkaline Gel Electrophoresis
  • The Comet Assay
  • Fast Micromethod DNA Single-Strand-Break Assay
  • 32P-Postlabeling DNA Damage Assays
  • Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA
  • Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision
  • Characterization of Enzymes That Initiate Base Excision Repair at Abasic Sites
  • Base Excision Repair in Mammalian Cells
  • In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts
  • Biochemical Assays for the Characterization of DNA Helicases
  • Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA
  • Assaying for the Dual Incisions of Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site
  • Analysis of Proliferating Cell Nuclear Antigen (PCNA) Associated With DNA Excision Repair Sites in Mammalian Cells
  • Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates.