Endocannabinoid Signaling : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Maccarrone, Mauro. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2016.
Edition:1st ed. 2016.
Series:Methods in Molecular Biology, 1412
Subjects:
Table of Contents:
  • Need for Methods to Investigate Endocannabinoid Signaling
  • Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
  • Determination of 2-Arachidonoylglycerol by µSPE-LC-MS/MS
  • Analysis of Omega-3 Fatty Acid Derived N-acylethanolamines in Biological Matrices
  • Assay of CB1 Receptor Binding
  • The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
  • Assay of TRPV1 Receptor Signaling
  • A Functional Assay for GPR55: Envision Protocols
  • The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
  • Assay of GTPγS Binding in Autoradiography
  • Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors
  • Assay of NAT Activity
  • Assay of NAPE-PLD Activity
  • Assay of FAAH Activity
  • Assay of NAAA Activity
  • Assay of DAGLα/β Activity
  • Assay of Monoacylglycerol Lipase Activity
  • A Sensitive and Versatile Fluorescent Activity Assay for ABHD6
  • A Sensitive and Versatile Fluorescent Activity Assay for ABHD12
  • Assay of Endocannabinoid Uptake
  • Assay of Endocannabinoid Oxidation by Cyclooxygenase-2
  • Oxygenation of Anandamide by Lipoxygenases
  • Assay of Endocannabinoid Oxidation by Cytochrome P450
  • Assessing Gene Expression of the Endocannabinoid System
  • Western Blotting of the Endocannabinoid System
  • Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells
  • Measuring ECS Interaction with Biomembranes
  • Visualization of Endocannabinoids in the Cell.