Directed Evolution Library Creation : Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | , , |
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Springer,
2014.
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| Edition: | 2nd ed. 2014. |
| Series: | Methods in Molecular Biology,
1179 |
| Subjects: |
Table of Contents:
- Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolution
- Error-Prone Rolling Circle Amplification Greatly Simplifies Random Mutagenesis
- Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli
- The Sequence Saturation Mutagenesis (SeSaM) Method
- Generation of Effective Libraries by Neutral Drift
- Site Saturation Mutagenesis
- Iterative Saturation Mutagenesis (ISM): A Powerful Approach to Engineer Proteins by Systematically Simulating Darwinian Evolution
- Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR)
- OmniChange: Simultaneous Site-Saturation of up to Five Codons
- Random Insertional-Deletional Strand Exchange Mutagenesis (RAISE): A Simple Method for Generating Random Insertion and Deletion Mutations
- Transposon-Based Approaches for Generating Novel Molecular Diversity during Directed Evolution
- Restriction Enzyme-Mediated DNA Family Shuffling
- Assembly of Designed Oligonucleotides (ADO): A Useful Tool in Synthetic Biology for Creating High Quality Combinatorial DNA Libraries
- One-Pot, Simple Methodology for Cassette Randomization and Recombination for Focused Directed Evolution (OSCARR)
- USER Friendly DNA Recombination (USERec): Gene Library Construction Requiring Minimal Sequence Homology
- ITCHY: Incremental Truncation for the Creation of Hybrid Enzymes
- Generating Random Circular Permutation Libraries
- Probabilistic Methods in Directed Evolution: Library Size, Mutation Rate and Diversity
- The Mutagenesis Assistant Program
- Computational Tools for Designing Smart Libraries
- Computational Tools for Directed Evolution: A Comparison of Prospective and Retrospective Strategies
- Designing Libraries of Chimeric Proteins Using SCHEMA Recombination and RASPP
- Non-Contiguous SCHEMA Protein Recombination
- Engineering Proteins by Reconstructing Evolutionary Adaptive Paths.