Ebolaviruses : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Hoenen, Thomas. (Editor ), Groseth, Allison. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1628
Materias:
Tabla de Contenidos:
  • Marburg and Ebola Viruses: A Look Back and Lessons for the Future
  • 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies
  • Ebolavirus: An Overview of Molecular and Clinical Pathogenesis
  • Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms
  • Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins
  • Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems
  • Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation
  • Modeling Ebolavirus Budding with Virus-Like Particles
  • Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays
  • Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins
  • Non-Radioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA
  • A Semi-Automated High Throughput Microtitration Assay for Filoviruses
  • Generation of Recombinant Ebola Viruses Using Reverse Genetics
  • Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals
  • Live-Cell Imaging of Filoviruses
  • Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds
  • Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence
  • Analyzing Apoptosis Induction and Evasion in Ebola Virus-Infected Cells
  • Electron Microscopy of Ebola Virus-Infected Cells
  • Validating the Inactivation Effectiveness of Chemicals on Ebola Virus
  • Visualizing Ebolavirus Particles Using Single-Particle Interferometric Reflectance Imaging Sensor (SP-IRIS)
  • Assessing Antiviral Countermeasures Using Mouse Models of Ebolavirus Infection
  • Evaluation of Ebola Virus Countermeasures in Guinea Pigs
  • Evaluation of Medical Countermeasures against Ebolaviruses in Non-Human Primate Models
  • Quantification of Filovirus Glycoprotein-Specific Antibodies
  • Monitoring Innate Immune Gene Responses in the Hamster Model of Ebola Virus Disease by RT-PCR
  • Real-Time and End Point PCR Diagnostics for Ebola Virus
  • Production of Antigens for ELISA
  • ELISA Methods for the Detection of Ebolavirus Infection
  • Ebola Virus Field Sample Collection.