DNA Damage Detection In Situ, Ex Vivo, and In Vivo : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Didenko, Vladimir V. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 682
Materias:
Tabla de Contenidos:
  • In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques.-Combination of TUNEL Assay with Immunohistochemistry for Simultaneous Detection of DNA Fragmentation and Oxidative Cell Damage
  • EM-ISEL: A Useful Tool to Visualize DNA Damage at the Ultrastructural Level
  • In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase
  • In Situ Ligation: a Decade and a Half of Experience.-In Situ Ligation Simplified: Using PCR Fragments for Detection of Double-Strand DNA Breaks in Tissue Sections
  • 5’OH DNA Breaks in Apoptosis and their Labeling by Topoisomerase-Based Approach
  • Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry
  • Fluorochrome-Labeled Inhibitors of Caspases (FLICA): Convenient In Vitro and In Vivo Markers of Apoptotic Cells for Cytometric Analysis
  • Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair. Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH)
  • Co-Localization of DNA Repair Proteins with UV-induced DNA Damage in Locally Irradiated Cells
  • Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects at High and Low-Frequencies. - Quantifying Etheno-DNA Adducts in Human Tissues, White Blood Cells and Urine by Ultrasensitive 32P-postlabeling and Immunohistochemistry
  • ELISpot Assay as a Tool to Study Oxidative Stress in Peripheral Blood Mononuclear Cells
  • Cytokinesis-Block Micronucleus Cytome assay in Lymphocytes
  • Buccal Micronucleus Cytome Assay
  • -H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts and Skin
  • Immunologic Detection of Benzo(a)pyrene-DNA Adducts
  • Non-Invasive Assessment of Oxidatively Damaged DNA: Liquid Chromatography-Tandem Mass Spectrometry Analysis of Urinary 8-Oxo-7,8-dihydro-2’-deoxyguanosine
  • Assessing sperm DNA fragmentation with the Sperm Chromatin Dispersion (SCD) test.