Diabetes Mellitus /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2003.
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| Edición: | 1st ed. 2003. |
| Colección: | Methods in Molecular Medicine,
83 |
| Materias: |
Tabla de Contenidos:
- Insulin Production
- Isolation of Islets of Langerhans from Rodent Pancreas
- Purification of Rat Pancreatic ?-Cells by Fluorescence-Activated Cell Sorting
- Assessment of Insulin Secretion in the Mouse
- Detection of Insulin Production by Immunohistochemistry
- Quantification of the Level of Insulin Gene Expression
- Chromatin Immunoprecipitation Using Isolated Islets of Langerhans
- Adenoviral Gene Transfer into ?-Cell Lines
- Utilization of NOD Mice in the Study of Type 1 Diabetes
- Insulin Action
- of DNA into 3T3-L1 Adipocytes by Electroporation
- Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes
- Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes
- Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay
- Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates
- Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity
- Assaying AKT/Protein Kinase B Activity
- Measurements of Cellular Phosphoinositide Levels in 3T3-L1 Adipocytes
- Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytes
- Measurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal Muscle
- Single Embryo Measurement of Basal-and Insulin-Stimulated Glucose Uptake
- Immunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose Transporters.