Genome Instability : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Muzi-Falconi, Marco. (Editor ), Brown, Grant W. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1672
Materias:
Tabla de Contenidos:
  • The A-like Faker Assay for Measuring Yeast Chromosome III Stability
  • The Chromosome Transmission Fidelity Assay for Measuring Chromosome Loss in Yeast
  • Measuring Mutation Rates using the Luria-Delbrück Fluctuation Assay
  • Molecular Genetic Characterization of Mutagenesis Using a Highly Sensitized Single-Stranded DNA Reporter System in Budding Yeast
  • Analyzing Genome Rearrangements in Saccharomyces cerevisiaen: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">
  • High Resolution Mapping of Modified DNA Nucleobases using Excision Repair Enzymes
  • Integrated Microarray-based Tools for Detection of Genomic DNA Damage and Repair Mechanisms
  • Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics
  • Inserting Site-specific DNA Lesions into Whole Genomes
  • A qPCR-based Protocol to Quantify DSB Resection
  • Alkaline Denaturing Southern Blot Analysis to Monitor Double-strand Break Processing
  • Single Molecule Analysis of Resection Tracks
  • Mapping DNA Breaks by Next-generation Sequencing
  • Genome-wide Profiling of DNA Double-strand Breaks by the BLESS and BLISS Methods
  • DNA Replication Profiling using Deep Sequencing
  • Quantitative Bromodeoxyuridine Immunoprecipitation Analyzed by High-Throughput Sequencing (qBrdU-seq or QBU)
  • Strand-specific Analysis of DNA Synthesis and Proteins Association with DNA Replication Forks in Budding Yeast
  • Analysis of Replicative Polymerase Usage by Ribonucleotide Incorporation
  • Dynamic Architecture of Eukaryotic DNA Replication Forks in vivo, Visualized by Electron Microscopy
  • A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation
  • Single Cell Gel Electrophoresis for the Detection of Genomic Ribonucleotides
  • Measuring the Levels of Ribonucleotides Embedded in Genomic DNA
  • Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing
  • Detection of DNA-RNA Hybrids in vivo
  • Analysis of de novo Telomere Addition by Southern Blot
  • Assays to Study Repair of Inducible DNA Double Strand Breaks at Telomeres
  • Telomerase RNA Imaging in Budding Yeast and Human Cells by Fluorescent in situ Hybridization
  • Methods to Study Repeat Fragility and Instability in Saccharomyces cerevisiae
  • Quantitative Analysis of the Rates for Repeat-mediated Genome Instability in a Yeast Experimental System
  • Measuring Dynamic Behaviour of Trinucleotide Repeat Tracts in vivo in Saccharomyces cerevisiae
  • The Detection and Analysis of Chromosome Fragile Sites
  • Imaging of DNA Ultrafine Bridges in Budding Yeast
  • Detection of Ultra-Fine Anaphase Bridges
  • A Chromatin Fiber Analysis Pipeline to Model DNA Synthesis and Structures in Fission Yeast
  • Long-term Imaging of DNA Damage and Cell Cycle Progression in Budding Yeast Using Spinning Disk Confocal Microscopy
  • The CellClamper: A Convenient Microuidic Device for Time-Lapse Imaging of Yeast
  • Characterization of Structural and Configurational Properties of DNA by Atomic Force Microscopy
  • Genome-wide Quantitative Fitness Analysis (QFA) of Yeast Cultures
  • Rewiring the Budding Yeast Proteome using Synthetic Physical Interactions
  • Reporter-based Synthetic Genetic Array Analysis: a Functional Genomics Approach for Investigating Transcript or Protein Abundance Using Fluorescent Proteins in Saccharomyces cerevisiae
  • Statistical Analysis and Quality Assessment of ChIP-seq Data with DROMPA
  • Quantitative Analysis of DNA Damage Signaling Responses to Chemical and Genetic Perturbations.