DNA Recombination : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Tsubouchi, Hideo. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 745
Materias:
Tabla de Contenidos:
  • Methods to Study Mitotic Homologous Recombination and Genome Stability
  • Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks and Telomere Ends
  • Characterization of Meiotic Recombination Initiation Sites Using Pulsed-field Gel Electrophoresis
  • Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-stranded DNA
  • Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes
  • Molecular Assays to Investigate Chromatin Changes during DNA Double-Strand Break Repair in Yeast
  • Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresis
  • Mapping of Crossover Sites Using DNA Microarrays
  • Using the Semi-Synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Budding Yeast Kinase, Mek1
  • Genetic and Molecular Analysis of Mitotic Recombination in Saccharomyces cerevisiae
  • In vivo Site-Specific Mutagenesis and Gene Collage Using the Delitto Perfetto System in Yeast Saccharomyces cerevisiae
  • Detection of RNA-Templated Double-Strand Break Repair in Yeast
  • SNP-Based Mapping of Crossover Recombination in C. elegans
  • Characterization of Meiotic Crossovers in Pollen from Arabidopsis thaliana
  • Isolation of Meiotic Recombinants from Mouse Sperm
  • Homologous Recombination Assay for Interstrand Crosslink Repair
  • Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line, DT40
  • Understanding the Immunoglobulin Locus Specificity of Hypermutation
  • Quality Control of Purified Proteins Involved in Homologous Recombination
  • Assays for Structure-Selective DNA Endonucleases
  • In vitro Assays for DNA Pairing and  Recombination-Associated DNA Synthesis
  • An In vitro Assay for Monitoring the Formation and Branch Migration of Holliday Junctions Mediated by a Eukaryotic Recombinase
  • Reconstituting the Key Steps of the DNA Double-Strand Break Repair In vitro
  • Biochemical Studies on Human Rad51-Mediated Homologous Recombination
  • Studying DNA Replication Fork Stability in Xenopus Egg Extract
  • Supported Lipid-Bilayers and DNA Curtains for High-Throughput Single Molecule Studies
  • FRET-Based Assays to Monitor DNA Binding and Annealing by Rad52 Recombination Mediator Protein
  • Visualization of Human Dmc1 Presynaptic Filaments
  • Tracking of Single and Multiple Genomic Loci in Living Yeast Cells
  • Cell Biology of Homologous Recombination in Yeast
  • Live Cell Imaging of Meiotic Chromosome Dynamics in Yeast
  • Chromosome Structure and Homologous Chromosome Association during Meiotic Prophase in C. elegans.