Exocytosis and Endocytosis /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Ivanov, Andrei I. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2014.
Edition:2nd ed. 2014.
Series:Methods in Molecular Biology, 1174
Subjects:
Table of Contents:
  • Pharmacological Inhibitors of Exocytosis and Endocytosis:  Novel Bullets for Old Targets
  • Systematic Analysis of Endocytosis by Cellular Perturbations
  • Real Time Detection of SNARE Complexes with FRET Using the Tetracysteine System
  • Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides
  • Secretion of Circular Proteins Using Sortase
  • Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-Epithelial Cells by OptiPrepTM Density Gradient Ultracentrifugation
  • Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis
  • Probabilistic Density Maps to Study the Spatial Organization of Endocytosis
  • Use of Kaede and Kikume Green-Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors
  • Halo Tag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells
  • SNAP-Tag to Monitor Trafficking of  Membrane Proteins in Polarized Epithelial Cells
  • FLAsH-PALM:  Super-Resolution Pointillist Imaging with FLAsH-tetracysteine Labeling
  • Analysis of Protein Dynamics with Tandem Fluorescent Protein Timers
  • Synchronization of Secretory Cargo Trafficking in Populations of Cells
  • Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins
  • A Method to Rapidly Induce Organelle-specific Molecular Activities and Membrane Tethering
  • A Novel Pair of Split Venus Fragments to Detect Protein-Protein Interactions by In Vitro and In Vivo Biomolecular Fluorescence Complementation Assays
  • Real Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy
  • Nanocones to Study Initial Steps of Endocytosis
  • A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography
  • VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy
  • Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network
  • Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans
  • Visualization of Clathrin-Mediated Endocytosis in Live Drosophila Egg Chambers
  • Novel Extraction Protocol to Probe the Role of Cholesterol in Synaptic Vesicle Recycling
  • Microfluidic Devices for Imaging Trafficking Events In Vivo Using Genetic Model Organisms
  • The "In Situ" Proximity Ligation Assay to Probe Protein-Protein Interactions in Intact Tissues
  • Probing the Role of the Actin Cytoskeleton During Regulated Exocytosis by Intravital Microscopy
  • Measurement of Dynamic F-actin Changes During Exocytosis.