Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Wajapeyee, Narendra. (Editor ), Gupta, Romi. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1507
Materias:
Tabla de Contenidos:
  • Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators
  • Tandem Affinity Purification Approach Coupled To Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-binding Proteins
  • Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos
  • Systematic Discovery Of Chromatin-bound Protein Complexes from ChIP-seq Datasets
  • Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) Protocol for Zebrafish Embryos
  • Establishment of time- and cell-specific RNAi in Caenorhabditis elegans
  • Cell-penetrating Peptide-mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing
  • Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets
  • eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response
  • High Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA using 4-thiouridine
  • Accurate Detection of Differential Expression and Splicing using Low-level Features
  • Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry
  • Determining if an mRNA is a Substrate of Nonsense-mediated mRNA Decay in Saccharomyces cerevisiae
  • Optimizing In Vitro Pre-mRNA 3' Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract
  • Unbiased Interrogation of 3D Genome Topology using Chromosome Conformation Capture Coupled to High-throughput Sequencing (3C-Seq)
  • Using an Inducible CRISPR-Cas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells
  • In vitro assay to Study Histone Ubiquitination during Transcriptional Regulation
  • Determination of Alternate Splicing Events using Transcriptome Arrays
  • Large-scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor
  • GeneTranscriptional Analysis-based Integrative Genomics Approach to Identify Tumor Promoting Metabolic Genes. .