DNA Topoisomerases : Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edition: | 1st ed. 2009. |
| Series: | Methods in Molecular Biology,
582 |
| Subjects: |
Table of Contents:
- 1. Introduction: Emerging Themes in DNA Topoisomerase Research
- 2. Analysis of DNA Topoisomers, Knots, and Catenanes by Agarose Gel Electrophoresis
- 3. Two-Dimensional Agarose Gel Electrophoresis of DNA Topoisomers
- 4. Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II
- 5. Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA
- 6. Monitoring the Topoisomerase II DNA Gate Conformational Change with Fluorescence Resonance Energy Transfer
- 7. Single-Molecule Magnetic Tweezers Studies of Type IB Topoisomerases
- 8. Dissolution of Double Holliday Junctions by the Concerted Action of BLM and Topoisomerase III?
- 9. ChIP-on-Chip Analysis of DNA Topoisomerases
- 10. Analyzing Top2 Distribution on Yeast Chromosomes by Chromatin Immunoprecipitation
- 11. Binding of DNA Topoisomerases I and II to Replication Origins
- 12. Cytometric Assessment of DNA Damage Induced by DNA Topoisomerase Inhibitors
- 13. Analysis of the Topoisomerase II-Dependent Decatenation G2 Checkpoint and Checkpoint Kinases in Human Cells
- 14. Assaying Topoisomerase II Checkpoints in Yeast
- 15. Cytological Analysis of Chromosome Structural Defects that Result from Topoisomerase II Dysfunction
- 16. Top2 SUMO Conjugation in Yeast Cell Lysates
- 17. Analysis of SUMOylation of Topoisomerase II? with Xenopus Egg Extracts
- 18. The Dynamics of DNA Topoisomerase II? in Living Cells
- 19. Depletion and Mutation of Topoisomerase II in Animal Cells.