High-Throughput Protein Production and Purification : Methods and Protocols /
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| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
2025 |
| Materias: |
Tabla de Contenidos:
- Part 1: General procedures for high-throughput protein production
- Overview of high-throughput cloning methods for the post-genomic era
- Overview of a high-throughput pipeline for streamlining the production of recombinant proteins
- Semi-automated small-scale purification method for high-throughput expression analysis of recombinant proteins
- High-Throughput Protein Production in Yeast
- A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells
- A High-throughput Automated Protein Folding System
- Part 2: High-throughput Protocols adapted to the production of specific protein families
- High-throughput production of oxidized animal toxins in Escherichia coli
- High-Throughput purification of protein kinases in Escherichia coli and insect cells
- Parallelized micro-scale expression of soluble scFv
- High throughput production of influenza virus-like particle (VLP) array by using VLP-factoryTM, a MultiBac baculoviral genome customized for enveloped VLP expression
- High-throughput Protein Production of membrane proteins in Saccharomyces cerevisiae
- High-throughput E. coli cell-free expression: From PCR product design to functional validation of GPCR
- High-Throughput Site-Directed Mutagenesis
- Part 3: High-throughput protein production combined with High-throughput micro-characterization for protein stability, solubility, quality and interactions
- Hot CoFi blot - A High-throughput colony-based screen for identifying more thermally stable protein variants
- High-throughput isolation of soluble protein domains using the bipartite split-GFP system
- High-throughput Analytical Light Scattering for Protein Quality Control and Characterization
- High-throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography
- A Flexible and Scalable High-Throughput Platform for Recombinant Membrane Protein Production
- Adaption of the Leishmania cell-free expression system to high-throughput analysis of protein interactions
- High-throughput protein-protein interaction assays using tripartite split-GFP complementation
- High-throughput production of a new library of human single and tandem PDZ domains allows quantitative PDZ-peptide interaction screening through high throughput holdup assay
- High throughput protein analysis using Negative Stain Electron Microscopy and 2D classification
- High-throughput protein production combined with High throughput SELEX identifies an extensive atlas of Ciona robusta transcription factor DNA-binding specificities
- High-throughput Micro-characterization of RNA-protein interactions.