Microbial Metabolic Engineering : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Santos, Christine Nicole S. (Editor ), Ajikumar, Parayil Kumaran. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1927
Materias:
Tabla de Contenidos:
  • Pathway and Gene Discovery from Natural Hosts and Organisms
  • Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity
  • Antisense RNA Elements for Downregulating Expression
  • Chemically Inducible Chromosomal Evolution (CIChE) for Multi-copy Metabolic Pathway Engineering
  • Bacterial Expression of Membrane Associated Cytochrome P450s and their Activity Assay in Nanodiscs
  • Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae
  • Assembly of Complex Pathways using Type IIs Restriction Enzymes
  • Combinatorial Assembly of Multigene Pathways by Combining Single Strand Assembly with Golden Gate Assembly
  • Checks and Balances with Use of the Keio Collection for Phenotype Testing
  • Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp. PCC 6803
  • Genetic Tools for Streamlined and Accelerated Pathway Engineering in Yarrowia lipolytica
  • Gas Chromatography-mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization
  • Targeted Mass Spectrometry-based Proteomics Tools for Strain Optimization
  • A Protocol for the Construction and Curation of Genome-scale Integrated Metabolic and Regulatory Network Models
  • 13C-Fingerprinting and Metabolic Flux Analysis of Microbial Metabolisms
  • A Genetic Screen for the Isolation of Mutants with Increased Flux in the Isoprenoid Pathway of Yeast.