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02565nam a22003135i 4500 |
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20210517153608.0 |
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110719s2011 xxu| s |||| 0|eng d |
| 020 |
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|a 9781617792526
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| 024 |
7 |
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|a 10.1007/978-1-61779-252-6
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Microtubule Dynamics :
|b Methods and Protocols /
|c edited by Anne Straube.
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| 250 |
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|a 1st ed. 2011.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2011.
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| 300 |
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|a XI, 319 p. 56 illus., 13 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 777
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| 505 |
0 |
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|a How to Measure Microtubule Dynamics? -- Purification of Tubulin from Porcine Brain -- Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe -- Generation of Differentially Polyglutamylated Microtubules -- Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms -- ab-Tubulin and Microtubule Binding Assays -- Analysis of Microtubule Assembly Kinetics Using Turbidimetry -- Isolation of Centrosomes from Cultured Cells -- Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds -- Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy -- Force Generation by Dynamic Microtubules In Vitro -- Variations on the Single Molecule Assay for Microtubule-Associated Proteins and Kinesins -- Analyzing the ATP Hydrolysis Cycle of Microtubule Motors -- Cryo-Electron Tomography of Microtubules Assembled In Vitro from Purified Components -- Kinetochore Microtubules, Analyzed by Correlated Light and Immuno-Electron Microscopy -- Photoactivatable-GFP-a-Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells -- Quantification of Asymmetric Microtubule Nucleation at Sub-Cellular Structures -- Investigating Microtubule Dynamic Instability Using Microtubule Targeting Agents -- L Isolation, Identification, and Validation of Microtubule-Associated Proteins from Drosophila Embryos -- Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Cell physiology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Cell Physiology.
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| 700 |
1 |
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|a Straube, Anne.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|