Microtubule Dynamics : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edition: | 1st ed. 2011. |
| Series: | Methods in Molecular Biology,
777 |
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Table of Contents:
- How to Measure Microtubule Dynamics?
- Purification of Tubulin from Porcine Brain
- Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe
- Generation of Differentially Polyglutamylated Microtubules
- Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms
- ab-Tubulin and Microtubule Binding Assays
- Analysis of Microtubule Assembly Kinetics Using Turbidimetry
- Isolation of Centrosomes from Cultured Cells
- Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds
- Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy
- Force Generation by Dynamic Microtubules In Vitro
- Variations on the Single Molecule Assay for Microtubule-Associated Proteins and Kinesins
- Analyzing the ATP Hydrolysis Cycle of Microtubule Motors
- Cryo-Electron Tomography of Microtubules Assembled In Vitro from Purified Components
- Kinetochore Microtubules, Analyzed by Correlated Light and Immuno-Electron Microscopy
- Photoactivatable-GFP-a-Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells
- Quantification of Asymmetric Microtubule Nucleation at Sub-Cellular Structures
- Investigating Microtubule Dynamic Instability Using Microtubule Targeting Agents
- L Isolation, Identification, and Validation of Microtubule-Associated Proteins from Drosophila Embryos
- Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques.