Histone Variants : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Orsi, Guillermo A. (Editor ), Almouzni, Geneviève. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1832
Materias:
Tabla de Contenidos:
  • Methods for Preparing Nucleosomes Containing Histone Variants
  • Characterization of Post-Translational Modifications on Histone Variants
  • Purification of Histone Variant-Interacting Chaperone Complexes
  • Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics
  • Histone Native Chromatin Immunprecipitation
  • How to Tackle Challenging ChIP-seq, with Long-Range Cross-Linking, using ATRX as an Example
  • Time-ChIP : A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance
  • MINCE-seq : Mapping In Vivo Nascent Chromatin with EdU and Sequencing
  • RChIP-seq : Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes
  • Bioinformatics Analysis of Nucleosome and Histone Variant Positioning
  • Imaging newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System
  • Real Time de novo Deposition of Centromeric Histone-Associated Proteins Using the Auxin Inducible Degradation System
  • Live Imaging of Parental Histone Variant Dynamics in UVC-Damaged Chromatin
  • CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A
  • Studying the Evolution of Histone Variants Using Phylogeny
  • Characterization of Post-Meiotic Male Germ Cell Genome Organizational States
  • An Animal Model for Genetic Analysis of Multi-Gene Families : Cloning and Transgenesis of Large Tandemly-Repeated Histone Gene Clusters
  • Imaging and Quantitation of Assembly Dynamics of the Centromeric Histone H3 Variant CENP-A in Drosophila melanogaster Spermatocytes by Immunofluorescence and Fluorescence In-Situ Hybridization (Immuno-FISH).