Intracellular Lipid Transport : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edition: | 1st ed. 2019. |
| Series: | Methods in Molecular Biology,
1949 |
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Table of Contents:
- 1. Analysis of Phosphatidylinositol Transfer at ER-PM Junctions in Receptor-stimulated Live Cells
- 2. Monitoring non-vesicular transport of phosphatidylserine and phosphatidylinositol 4-phosphate in intact cells by BRET analysis
- 3. Development of non-specific BRET-based biosensors to monitor plasma membrane inositol lipids in living cells
- 4. Following anterograde transport of phosphatidylserine in yeast in real time
- 5. Imaging lipid metabolism at the Golgi complex
- 6. Advanced in vitro assay system to measure PS and PE transport at ER/mitochondria interface
- 7. Measurement of lipid transport in mitochondria by the MTL complex
- 8. Bi- and trifunctional lipids for visualization of sphingolipid dynamics within the cell
- 9. Indirect lipid transfer protein activity measurements using quantification of glycosphingolipid production
- 10. Measurement of intracellular sterol transport in yeast
- 11. Intracellular and plasma membrane cholesterol labeling and quantification using filipin and GFP-D4
- 12. Monitoring and modulating intracellular cholesterol trafficking using ALOD4, a cholesterol-binding protein
- 13. Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts
- 14. Preparation of proteoliposomes with purified TMEM16 protein for accurate measures of lipid scramblase activity
- 15. In vitro assays to measure the membrane tethering and lipid transport activities of the Extended Synaptotagmins
- 16. Determining the lipid binding specificity of SMP domains: an ERMES subunit as a case study
- 17. In Vitro Measurement of Sphingolipid Intermembrane Transport Illustrated by GLTP Superfamily Members
- 18. Purification and Characterization of Human Niemann-Pick C1 Protein
- 19. In vitro strategy to measure sterol/phosphatidylinositol 4-phosphate exchange between membranes
- 20. Determination of Ligand Binding Affinity and Specificity of Purified START Domains by Thermal Shift Assays Using Circular Dichoism
- 21. Synthesis of Fluorescent Membrane-spanning Lipids for Studies of Lipid Transfer and Membrane-Fusion
- 22. Setting-up all-atom molecular dynamics simulations to study the interactions of peripheral membrane proteins with model lipid bilayers. .