Microbial Proteomics : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Becher, Dörte. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1841
Materias:
Tabla de Contenidos:
  • Filter-Aided Sample Preparation for Proteome Analysis
  • Protein Enrichment from Highly Dilute Samples with StrataClean
  • Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins
  • Enrichment of Cell Surface-Associated Proteins in Gram+ Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis
  • Preparation of Bacterial Magnetosomes for Proteome Analysis
  • Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics
  • Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS
  • Applications of Difference Gel Electrophoresis (DIGE) on the Study of Microorganisms
  • Proteomic Signatures in Staphylococcus aureus
  • How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis
  • Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE
  • Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses
  • Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)
  • Next-Generation Trapping of Protease Substrates by Label-Free Proteomics
  • In Vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host-Pathogen Settings
  • Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography
  • Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation
  • Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria
  • Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host-Microbiota Interactions
  • Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis / Proteome Extraction from Soil Samples for Metaproteomics Studies
  • Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter
  • Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria-Invertebrate Symbioses.