Horizontal Gene Transfer : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer US : Imprint: Humana,
2020.
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| Edición: | 1st ed. 2020. |
| Colección: | Methods in Molecular Biology,
2075 |
| Materias: |
Tabla de Contenidos:
- Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples
- Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health
- Detection, Isolation, and Characterization of Plasmids in the Environment
- Bacteriophage Isolation and Characterization: Phages of Escherichia coli
- Detection and Characterization of Transposons in Bacteria
- Measuring Plasmid Conjugation Using Antibiotic Selection
- Measuring Plasmid Conjugation Using Fluorescent Reporters
- Methods to Quantify DNA Transfer in Enterococcus
- Quantifying and Characterizing Distributive Conjugal Transfer in Mycobacterium smegmatis
- Spectrophotometric Assays to Quantify the Activity of T4SS ATPases
- Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis
- First Biochemical Steps on Bacterial Transposition Pathways
- Natural Transformation in Escherichia coli
- Integron Identification in Bacterial Genomes and Cassette Recombination Assays
- Methods to Identify and Analyze Vesicle-Protected DNA Transfer
- Measuring Plasmid Stability in Gram-Negative Bacteria
- Methods for the Analysis and Characterization of Defence Mechanisms against Horizontal Gene Transfer: CRISPR Systems
- The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements
- Identifying Conjugative Plasmids and Integrative Conjugative Elements with CONJscan
- PlasmidFinder and In Silico pMLST: Identification and Typing of Plasmid Replicons in Whole Genome Sequencing (WGS)
- MOBscan: Automated Annotation of MOB Relaxases
- Plasmid Typing and Classification
- Plasmid Reconstruction from Next-Gen Data: A Detailed Protocol for the Use of PLACNETw for the Reconstruction of Plasmids from WGS Datasets
- Statistical Analysis of Accessory Genome
- Inferring Horizontal Gene Transfer with DarkHorse, Phylomizer, and ETE Toolkits
- Methods to Study Fitness and Compensatory Adaptation in Plasmid-Carrying Bacteria
- A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative Bacteria
- Conjugative Assembly Genome Engineering (CAGE).