HPLC of Peptides and Proteins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Aguilar, Marie-Isabel. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:1st ed. 2004.
Colección:Methods in Molecular Biology, 251
Materias:
Tabla de Contenidos:
  • Modes of HPLC
  • HPLC of Peptides and Proteins
  • Reversed-Phase High-Performance Liquid Chromatography
  • Ion-Exchange Chromatography
  • High-Performance Hydrophobic Interaction Chromatography
  • Gel Filtration Chromatography
  • Hydrophilic Interaction Chromatography
  • Immobilized Metal Ion Affinity Chromatography of Proteins
  • Immunoaffinity Chromatography of Proteins
  • Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins
  • Preparative Methodologies
  • Capillary Separations
  • Micropreparative HPLC of Peptides and Proteins
  • Multidimensional HPLC Purification of Proteins
  • Analytical High-Performance Liquid Chromatography
  • Prep/Semiprep Separations of Peptides
  • Large-Scale Protein Chromatography
  • Applications
  • Proteolytic Peptide Mapping
  • Mass Spectrometric Characterization of Posttranslationally Modified Proteins—Phosphorylation
  • Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry
  • HPLC in the Analysis of Peptide Metabolism
  • Isolation and Characterization of Naturally Processed MHC-Bound Peptides From the Surface of Antigen-Presenting Cells
  • Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques
  • HPLC and Mass Spectrometry of Intrinsic Membrane Proteins
  • IgG Purification
  • DNA-Binding Proteins
  • Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC
  • Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry
  • Proteome Analysis.