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100301s2009 xxu| s |||| 0|eng d |
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|a 9781597451963
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| 024 |
7 |
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|a 10.1007/978-1-59745-196-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a High Throughput Protein Expression and Purification :
|b Methods and Protocols /
|c edited by Sharon A. Doyle.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XII, 322 p. 57 illus., 6 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 498
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| 505 |
0 |
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|a High-Throughput Protein Production (HTPP): A Review of Enabling Technologies to Expedite Protein Production -- Designing Experiments for High-Throughput Protein Expression -- Gateway Cloning for Protein Expression -- Flexi Vector Cloning -- The Precise Engineering of Expression Vectors Using High-Throughput In-Fusion™ PCR Cloning -- The Polymerase Incomplete Primer Extension (PIPE) Method Applied to High-Throughput Cloning and Site-Directed Mutagenesis -- A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins -- "System 48" High-Throughput Cloning and Protein Expression Analysis -- Automated 96-Well Purification of Hexahistidine-Tagged Recombinant Proteins on MagneHis Ni2+-Particles -- E. coli and Insect Cell Expression, Automated Purification and Quantitative Analysis -- Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner for the Production of Soluble Recombinant Proteins in Escherichia coli -- PHB-Intein-Mediated Protein Purification Strategy -- High-Throughput Biotinylation of Proteins -- High-Throughput Insect Cell Protein Expression Applications -- High-Throughput Protein Expression Using Cell-Free System -- The Production of Glycoproteins by Transient Expression in Mammalian Cells -- High-Throughput Expression and Detergent Screening of Integral Membrane Proteins -- Cell-Free Expression for Nanolipoprotein Particles: Building a High-Throughput Membrane Protein Solubility Platform -- Expression and Purification of Soluble His6-Tagged TEV Protease -- High-Throughput Protein Concentration and Buffer Exchange: Comparison of Ultrafiltration and Ammonium Sulfate Precipitation.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Proteins .
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Protein Structure.
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| 700 |
1 |
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|a Doyle, Sharon A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|