Hypoxia : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook | 
| Lenguaje: | English | 
| Publicado: | 
      New York, NY :
        Springer New York : Imprint: Humana,
    
      2018.
     | 
| Edición: | 1st ed. 2018. | 
| Colección: | Methods in Molecular Biology,
              1742             | 
| Materias: | 
                Tabla de Contenidos: 
            
                  - Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma
 - Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina Bead Arrays
 - Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens
 - Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens
 - Isolation of GBM Stem Cells with Flow Cytometry
 - Lentiviral Transduction of Primary Human Glioblastoma Cultures
 - Selective Targeting of CD133-expressing Glioblastoma Stem Cells using Lentiviral Vectors
 - Intracellular pH Measurements in Glioblastoma Cells using the pH-sensitive Dye BCECF
 - Induction and Assessment of Hypoxia in Glioblastoma Cells in vitro
 - Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography-tandem Mass Spectrometry
 - Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS)
 - Single-Cell RNA Sequencing of Glioblastoma Cells
 - Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures
 - Orthotopic Patient-derived Glioblastoma Xenografts in Mice
 - Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice
 - Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy
 - Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection
 - Flow Cytometric Identification of Tumor-infiltrating Lymphocytes from Glioblastoma
 - Modeling Glioma with Human Embryonic Stem Cell-derived Neural Lineages. .