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100301s2002 xxu| s |||| 0|eng d |
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|a 9781592591947
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| 024 |
7 |
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|a 10.1385/1592591949
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a In Vitro Mutagenesis Protocols /
|c edited by Jeff Braman.
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| 250 |
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|a 2nd ed. 2002.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2002.
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| 300 |
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|a XVI, 288 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 182
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| 505 |
0 |
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|a Rapid and Reliable Site-Directed Mutagenesis Using Kunkel’s Approach -- Site-Directed Mutagenesis Using Altered /gb-Lactamase Specificity -- Site-Directed Mutagenesis Facilitated by DpnI Selection on Hemimethylated DNA -- Multiple Site-Directed Mutagenesis In Vitro -- Two-Stage Polymerase Chain Reaction Protocol Allowing Introduction of Multiple Mutations, Deletions, and Insertions, Using QuikChangeTM Site-Directed Mutagenesis -- Efficient and Accurate Site-Directed Mutagenesis of Large Plasmids -- Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis -- Site-Directed Mutagenesis Mediated by a Single Polymerase Chain Reaction Product -- Megaprimer Method for Polymerase Chain Reaction-Mediated Generation of Specific Mutations in DNA -- Generation of Epitope-Tagged Proteins by Inverse Polymerase Chain Reaction Mutagenesis -- Site-Directed Mutagenesis by Polymerase Chain Reaction -- Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction -- Phenotypic Expression of Polymerase Chain Reaction-Generated Random Mutations in a Foreign Gene After its Introduction into an Acinetobacter Chromosome by Natural Transformation -- Polymerase Chain Reaction-Mediated Mutagenesis in Sequences Resistant to Homogeneous Amplification -- Polymerase Chain Reaction-Based Signature-Tagged Mutagenesis -- High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction -- In Vitro Scanning-Saturation Mutagenesis -- Random Transposon Mutagenesis of Large DNA Molecules in Escherichia coli -- Random Chromosomal Gene Disruption Using Cassette Mutagenesis -- Transplacement Mutagenesis -- Preparation of Transposon Insertion Lines and Determination of Insertion Sites in Arabidopsis Genome -- Evolutionary Molecular Engineering by Random Elongation Mutagenesis -- Random Mutagenesis for Protein Breeding -- DNA Shuffling and Family Shuffling for In Vitro Gene Evolution -- Mutagenic Polymerase Chain Reaction of Protein-Coding Genes for In Vitro Evolution.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Braman, Jeff.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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