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|a 9781607616528
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| 024 |
7 |
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|a 10.1007/978-1-60761-652-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a In Vitro Mutagenesis Protocols :
|b Third Edition /
|c edited by Jeff Braman.
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| 250 |
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|a 3rd ed. 2010.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2010.
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| 300 |
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|a XIV, 442 p. 92 illus., 9 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 634
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| 505 |
0 |
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|a Mutagenesis in Various Microbial Backgrounds -- Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension -- In Vitro Mutagenesis of Brucella Species -- Random Mutagenesis Strategies for Campylobacter and Helicobacter Species -- Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes -- An Efficient Protocol for VZV BAC-Based Mutagenesis -- A Method for Rapid Genetic Integration into Plasmodium falciparum Utilizing Mycobacteriophage Bxb1 Integrase -- PCR Mutagenesis -- Random Mutagenesis by Error-Prone PCR -- A Rapid and Versatile PCR-Based Site-Directed Mutagenesis Protocol for Generation of Mutations Along the Entire Length of a Cloned cDNA -- Rapid Sequence Scanning Mutagenesis Using In Silico Oligo Design and the Megaprimer PCR of Whole Plasmid Method (MegaWHOP) -- Insertion and Deletion Mutagenesis by Overlap Extension PCR -- Targeted Amplification of Mutant Strands for Efficient Site-Directed Mutagenesis and Mutant Screening -- A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated Proteins -- Amplification of Orthologous Genes Using Degenerate Primers -- Reviews -- Computational Evaluation of Protein Stability Change upon Mutations -- Approaches for Using Animal Models to Identify Loci That Genetically Interact with Human Disease-Causing Point Mutations -- Protein Evolution Mutagenesis -- Using Peptide Loop Insertion Mutagenesis for the Evolution of Proteins -- Massive Mutagenesis®: High-Throughput Combinatorial Site-Directed Mutagenesis -- Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High-Throughput Screening -- Ribosome Display for Rapid Protein Evolution by Consecutive Rounds of Mutation and Selection -- Protein Structure and Function Mutagenesis -- Fine-Tuning Enzyme Activity Through Saturation Mutagenesis -- Characterization of Structural Determinants of Type 1 Corticotropin Releasing Hormone (CRH) Receptor Signalling Properties -- Site-Directed Mutagenesis for Improving Biophysical Properties of VH Domains -- Phenotype Based Functional Gene Screening Using Retrovirus-Mediated Gene Trapping in Quasi-Haploid RAW 264.7 Cells -- Site-Directed Disulfide Cross-Linking to Probe Conformational Changes of a Transporter During Its Functional Cycle: Escherichia coli AcrB Multidrug Exporter as an Example -- Site-Specific Incorporation of Extra Components into RNA by Transcription Using Unnatural Base Pair Systems -- Random Mutagenesis -- Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases -- Random-Scanning Mutagenesis -- Easy Two-Step Method for Randomizing and Cloning Gene Fragments -- Mutator Bacterial Strain Mutagenesis -- Random Mutagenesis Using a Mutator Strain -- En Passant Mutagenesis: A Two Step Markerless Red Recombination System.
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| 650 |
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|a Genetic engineering.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
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0 |
|a Proteomics.
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| 650 |
1 |
4 |
|a Genetic Engineering.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Proteomics.
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| 700 |
1 |
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|a Braman, Jeff.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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