Hematopoietic Stem Cell Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Klug, Christopher A. (Editor ), Jordan, Craig T. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2002.
Edición:1st ed. 2002.
Colección:Methods in Molecular Medicine, 63
Materias:
Tabla de Contenidos:
  • Isolation and Analysis of Hematopoietic Stem Cells from Mouse Embryos
  • The Purification of Mouse Hematopoietic Stem Cells at Sequential Stages of Maturation
  • Flow Cytometry and Immunoselection of Human Stem Cells
  • Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
  • Noninvasive Measurement of Hematopoietic Stem Cell Cycling and Turnover by In Vivo Bromodeoxyuridine Incorporation
  • Isolation and Characterization of Primitive Hematopoietic Cells Based on Their Position in the Cell Cycle
  • Hematopoietic Colony-Forming Cells
  • Long-Term Culture-Initiating Cell Assays for Human and Murine Cells
  • The Cobblestone-Area-Forming Cell Assay
  • CFU-S
  • Intrathymic Injection for Analysis of T-Cell Progenitor Activity
  • Quantitation of Murine and Human Hematopoietic Stem Cells by Limiting-Dilution Analysis in Competitively Repopulated Hosts
  • Ex Vivo Expansion of Human and Murine Hematopoietic Stem Cells
  • Hematopoietic Development of ES Cells in Culture
  • Genetic Modification of Murine Hematopoietic Stem Cells by Retroviruses
  • Retroviral Transduction of FACS-Purified Hematopoietic Stem Cells
  • Retroviral-Mediated Transduction and Clonal Integration Analysis of Human Hematopoietic Stem and Progenitor Cells
  • Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
  • Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
  • Identification of Differentially Expressed Genes in Sorted Cell Populations by Two-Dimensional Gene-Expression Fingerprinting.