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150327s2015 ne | s |||| 0|eng d |
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|a 9789401797160
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|a 10.1007/978-94-017-9716-0
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Handbook of Vascular Biology Techniques /
|c edited by Mark Slevin, Garry McDowell.
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| 250 |
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|a 1st ed. 2015.
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|a Dordrecht :
|b Springer Netherlands :
|b Imprint: Springer,
|c 2015.
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| 300 |
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|a XII, 477 p. 191 illus., 126 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
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|a Preface -- Section 1 In vitro techniques -- In vitro angiogenesis assay - endothelial migration, proliferation, and tube formation -- Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis -- Induction of hypoxia in vascular endothelial cell culture -- Evaluating In Vitro Angiogenesis using Live Cell Imaging -- Isolation of Endothelial Progenitor Cells (EPCs) -- Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis -- Assessment of vascular function and contractility, ex vivo -- Measurement of Aβ uptake by cerebrovascular smooth muscle cells -- Measurement of Intracellular Ca2+ in Human Endothelial cells -- Section 2 In vivo and ex vivo manipulations -- Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion -- Sponge implant model of inflammatory angiogenesis -- The chick embryo chorioallantoic membrane assay -- Dorsal air sac assay -- Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals -- Hypoxia-induced retinal angiogenesis in adult zebrafish -- Angiogenesis in regenerating adult zebrafish tail fin -- Methods for studying developmental angiogenesis in zebrafish -- Isolation and Expansion of Brain Microvascular Endothelial Cells -- Section 3 Imaging and histological analysis -- Adipose angiogenesis -- Assessing tumor angiogenesis in histological samples -- Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea -- Computed Tomography Angiography: Fundamental techniques and data interpretation -- Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation -- Single-Photon Emission Tomography of the brain in vascular pathology -- Section 4 Miscellaneous novel techniques in vascular biology -- Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues -- Preparation of liposomes with dual fluorophores to follow real-time content release in vivo -- Vascular Flow Modelling using Computational Fluid Dynamics -- Reverse Transcription Real-Time PCR Protocol for gene expression analyses -- Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis -- Genomic microarray analysis -- Selection of appropriate housekeeping genes for quality control -- Endothelial transcriptomic analysis -- Protocol for Multiplex Amplicon Sequencing using Barcoded Primers -- Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells: Identification and Quantification -- A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood -- Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry.
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| 650 |
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|a Cancer research.
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| 650 |
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|a Biology—Technique.
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| 650 |
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|a Laboratory medicine.
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| 650 |
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|a Cancer Research.
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| 650 |
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4 |
|a Biological Techniques.
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| 650 |
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4 |
|a Laboratory Medicine.
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| 700 |
1 |
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|a Slevin, Mark.
|e editor.
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| 700 |
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|a McDowell, Garry.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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