Integrin and Cell Adhesion Molecules : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Shimaoka, Motomu. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edition:1st ed. 2012.
Series:Methods in Molecular Biology, 757
Subjects:
Table of Contents:
  • Overview: Assays for Studying Integrin-Dependent Cell Adhesion
  • Cell Adhesion Assays
  • Real Time Analysis of Integrin-dependent Transendothelial Migration and Integrin-independent Interstitial Motility of Leukocytes
  • Lentiviral Gene Transfer Method to Study Integrin Function in T Lymphocytes
  • Surface Plasmon Resonance Biosensing in Studies of the Binding between b2 Integrin I Domains and their Ligands
  • Cell-Free Ligand-Binding Assays for Integrin LFA-1
  • Overview: Structural Biology of Integrins
  • Protein Expression and Purification of Integrin I Domains and IgSF Ligands for Crystallography
  • Electron Microscopic Imaging of Integrin
  • An NMR Method to Study Protein-Protein Interactions
  • Single Molecule Methods to Study Cell Adhesion Molecules
  • Overview: Imaging in the Study of Integrins
  • Live Imaging of LFA-1-dependent T Cell Motility and Stop Signals
  • Monitoring Integrin Activation by Fluorescence Resonance Energy Transfer
  • High-resolution Fluorescence Microscopy to Study Transendothelial Migration
  • Multiphoton Intravital Microscopy to Study Lymphocyte Motility in Lymph Nodes
  • Overview of Integrin Signaling in the Immune System
  • Rap1 and Integrin Inside-out Signaling
  • Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis
  • Talin and Signaling Through Integrins
  • Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins
  • Overview: Studying Integrins In Vivo
  • A Method for the Generation of Conditional Gene-Targeted Mice
  • T Cell Homing to the Gut Mucosa: General Concepts and Methodological Considerations
  • In Vivo Quantitative Proteomics: the SILAC-Mouse
  • Analysis of Chemotaxis in Dictyostelium
  • Integrins in Tumor Angiogenesis & Lymphangiogenesis
  • PET-Radioimmunodetection of Integrins: Imaging Acute Colitis Using a 64Cu-Labeled Anti-b7 Integrin Antibody
  • Integrin-targeted Nanoparticles for siRNA Delivery.- Humanized Mice for Studying Human Leukocyte Integrins In Vivo.