In Vitro Mutagenesis : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edición: | 1st ed. 2017. |
| Colección: | Methods in Molecular Biology,
1498 |
| Materias: |
Tabla de Contenidos:
- Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells
- Mutagenesis and Genome Engineering of Epstein-Barr Virus in Cultured Human Cells by CRISPR/Cas9
- Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice
- All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells
- CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium
- Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii
- Generation of Stable Knockout Mammalian Cells By TALEN-Mediated Locus-Specific Gene Editing
- Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell- Mediated Semi-Cloned Technology. - Insertion of Group II Intron-Based Ribozyme Switches into Homing Endonuclease Genes
- Generating a Genome Editing Nuclease for Targeted Mutagenesis in Human Cells
- Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis
- in silico Approaches to Identify Mutagenesis Targets to Probe and Alter Protein-Cofactor and Protein-Protein Functional Relationships
- in silico Prediction of Deleteriousness for Non-Synonymous and Splice-Altering Single Nucleotide Variants in the Human Genome
- in silico methods for Analyzing Mutagenesis Targets
- Methods for Detecting Critical Residues in Proteins
- A Method for Bioinformatic Analysis of Transposon Insertion Sequencing (INSeq) Results for Identification of Microbial Fitness Determinants
- Application of in vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea
- Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors
- PERMutation Using Transposase Engineering (PERMUTE): a Simple Approach for Constructing Circularly-Permuted Protein Libraries
- Transposon Insertion Mutagenesis for Archaeal Gene Discovery
- Genome-Wide Transposon Mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis
- Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method
- Seamless Ligation Cloning Extract (Slice) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to Slip Site-Directed Mutagenesis
- Facile Site-Directed Mutagenesis of Large Constructs Using Gibson Isothermal DNA Assembly
- Revised Mechanism and Improved Efficiency of the Quikchange Site-Directed Mutagenesis Method
- An in vitro Single Primer Site-Directed Mutagenesis Method for Use in Biotechnology
- Use of Megaprimer and Overlapping Extension PCR (OE-PCR) to Site-Specifically Mutagenize and Enhance Cyclodextrin Glucosyltransferase (CGTase) Function
- Step-by-Step in vitro Mutagenesis - Lessons from Fucose-Binding Lectin PA-IIL
- Analytical Methods for Assessing the Effects of Site-Directed Mutagenesis on Protein-Cofactor and Protein-Protein Functional Relationships
- Biochemical and Biophysical Methods to Examine the Effects of Site-Directed Mutagenesis on Enzymatic Activities and Interprotein Interactions
- Use of Random and Site-Directed Mutagenesis to Probe Protein Structure-Function Relationships: Applied Techniques in the Study of Helicobacter pylori
- Novel Random Mutagenesis Method for Directed Evolution
- Random Mutagenesis by Error-Prone Polymerase Chain Reaction Using a Heavy Water Solvent
- Development and Use of a Novel Random Mutagenesis Method: in Situ Error-Prone PCR (is-epPCR).