MAP Kinase Signaling Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Seger, Rony. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edición:2nd ed. 2010.
Colección:Methods in Molecular Biology, 661
Materias:
Tabla de Contenidos:
  • Activation and Function of Components of the MAP Kinase Signaling Cascades
  • The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of Physiological Functions
  • Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation
  • Activation of SAPK/JNKs In Vitro
  • Activation of p38 and Determination of Its Activity
  • Activity Assays for Extracellular Signal-Regulated Kinase 5
  • Use of Inhibitors in the Study of MAP Kinases
  • Study of MAP Kinase Cascades as Transmitters of Membranal Receptor Signals
  • MAP Kinase Activation by Receptor Tyrosine Kinases: In Control of Cell Migration
  • Activation of Ras and Rho GTPases and MAP Kinases by G-Protein-Coupled Receptors
  • Regulation of MAP Kinase Signaling by Calcium
  • Identification of Novel Substrates of MAP Kinase Cascades Using Bioengineered Kinases that Uniquely Utilize Analogs of ATP to Phosphorylate Substrates
  • ERK-MAP Kinase Signaling in the Cytoplasm
  • Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases
  • Structure-Function Relationships and Localization of MAP Kinases
  • Structural Studies of MAP Kinase Cascade Components
  • Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry
  • A “Molecular Evolution” Approach for Isolation of Intrinsically Active (MEK-Independent) MAP Kinases
  • Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
  • Localization and Trafficking of Fluorescently Tagged ERK1 and ERK2
  • Studies on the Regulation of MAP Kinase Cascades
  • Studying the Regulation of MAP Kinase by MAP Kinase Phosphatases In Vitro and in Cell Systems
  • Proteomic Analysis of Scaffold Proteins in the ERK Cascade
  • Analysis of ERKs’ Dimerization by Electrophoresis
  • MAP Kinase: SUMO Pathway Interactions
  • Computational Modelling of Kinase Signalling Cascades
  • Use of Lower Organisms, Animal Models, and Human Genetics in the Study of MAP Kinases
  • Analysis of Mitogen-Activated Protein Kinase Activity in Yeast
  • Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining
  • Studying MAP Kinase Pathways During Early Development of Xenopus laevis
  • Deciphering Signaling Pathways In Vivo: The Ras/Raf/Mek/Erk Cascade
  • Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes
  • Study of MAP Kinases in Specific Physiological Systems
  • Implication of the ERK Pathway on the Post-transcriptional Regulation of VEGF mRNA Stability
  • Studies on MAP Kinase Signaling in the Immune System
  • Methods to Study MAP Kinase Signalling in the Central Nervous System
  • MAP Kinase Regulation of the Mitotic Spindle Checkpoint
  • Using High-Content Microscopy to Study Gonadotrophin-Releasing Hormone Regulation of ERK.