Mouse Oocyte Development : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Verlhac, Marie-Hélène. (Editor ), Terret, Marie-Emilie. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1818
Materias:
Tabla de Contenidos:
  • Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse
  • In vitro Culture of Mouse Oocytes for Meiotic Maturation
  • In vitro Maturation and Culture of Human Oocytes
  • Pronuclear Transfer in Human Oocytes
  • In vitro Fertilization of Mouse Oocyte
  • Profiling Maternal mRNA Translation during Oocyte Development
  • Transcriptome Profiling of Single Mouse Oocytes
  • Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation
  • Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation
  • Triple-colour Live Imaging of Mouse Oocytes
  • Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes
  • Manipulating Cohesin Levels in Live Mouse Oocytes
  • Optogenetic Manipulation of Mouse Oocytes
  • Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin
  • Photoactivation of Actin in Mouse Oocyte
  • Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes
  • Micropipette Aspiration of Oocytes to Assess Cortical Tension.