Mammalian Oocyte Regulation : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Homer, Hayden A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:1st ed. 2013.
Colección:Methods in Molecular Biology, 957
Materias:
Tabla de Contenidos:
  • Studying Recombination in Mouse Oocytes
  • Analysis of Recombinants in Female Mouse Meiosis
  • Studying Meiosis Specific Cohesins in Mouse Embryonic Oocytes
  • Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development
  • Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos
  • Time-Lapse Epi-Fluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oocytes
  • Using FRET to Study RanGTP Gradients in Live Mouse Oocytes
  • Making cRNA for Microinjection and Expression of Fluorescently-Tagged Proteins for Live-Cell Imaging in Oocytes
  • RNAi-Based Methods for Gene Silencing in Mouse Oocytes
  • Micro-Injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes
  • Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos
  • Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes
  • Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
  • Chromosome Spreads with Centromere Staining in Mouse Oocytes
  • Preparation of Mammalian Oocytes for Transmission Electron Microscopy
  • Measuring Ca2+ Oscillations in Mammalian Eggs
  • Counting Chromosomes in Intact Eggs
  • Free-Hand Bisection of Mouse Oocytes and Embryos
  • Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes
  • Nuclear Transfer in the Mouse Oocyte.