Hedgehog Signaling Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2007.
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| Edición: | 1st ed. 2007. |
| Colección: | Methods in Molecular Biology,
397 |
| Materias: |
Tabla de Contenidos:
- Purifying the Hedgehog Protein and its Variants
- Application of Sonic Hedgehog to the Developing Chick Limb
- Manipulation of Hedgehog Signaling in Xenopus by Means of Embryo Microinjection and Application of Chemical Inhibitors
- Isolation of Rat Telencephalic Neural Explants to Assay Shh GABAergic Interneuron Differentiation-Inducing Activity
- Genetic Analysis of the Vertebrate Hedgehog-Signaling Pathway Using Muscle Cell Fate Specification in the Zebrafish Embryo
- Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems
- Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry
- Detecting Tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for Functional Analysis
- Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent In Situ Hybridization of Hedgehog Target Genes
- RNAi in the Hedgehog Signaling Pathway: pFRiPE, a Vector for Temporally and Spatially Controlled RNAi in Drosophila
- Germline Clone Analysis for Maternally Acting Drosophila Hedgehog Components
- Clonal Analysis of Hedgehog Signaling in Drosophila Somatic Tissues
- GAL4/UAS Targeted Gene Expression for Studying Drosophila Hedgehog Signaling
- Biochemical Fractionation of Drosophila Cells
- Using Immunoprecipitation to Study Protein–Protein Interactions in the Hedgehog-Signaling Pathway
- Sequence Analyses to Study the Evolutionary History and Cis-Regulatory Elements of Hedgehog Genes.