Microbial Toxins : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edición: | 1st ed. 2011. |
| Colección: | Methods in Molecular Biology,
739 |
| Materias: |
Tabla de Contenidos:
- Bacterial Protein Toxins
- Detection of Bacterial Protein Toxins by Solid Phase Magnetic Immunocapture and Mass Spectrometry. Sensitive and Rapid Detection of Cholera Toxin-Producing Vibrio cholerae using Loop-Mediated Isothermal Amplification. Ultra Sensitive Detection of Botulinum Neurotoxins and Anthrax Lethal Factor in Biological Samples by ALISSA. Examination of Bacillus anthracis Spores by Multiparameter Flow Cytometry. A Cell-Based Fluorescent Assay to Detect the Activity of Shiga Toxin and Other Toxins That Inhibit Protein Synthesis. Use of a Vero Cell-Based Fluorescent Assay to Assess Relative Toxicities of Shiga Toxin 2 Subtypes from Escherichia coli. Molecular Methods – Chip Assay and Quantitative Real-Time PCR – in Detecting Hepatotoxic Cyanobacteria. Endotoxins
- Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes from Whole-Cell Lysates. Isolation of Smooth-Type Lipopolysaccharides to Electrophoretic Homogeneity. A Method for Unobtrusive Labeling of Lipopolysaccharides with Quantum Dots. Fluorescence-Based Methods to Assay Inhibitors of Lipopolysaccharide Synthesis. Micromethods for Lipid A Isolation and Structural Characterization. Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides (LPS) with Probes Bearing Hydrazine or Amino Groups. I. LPS Activation with Cyanogen Bromide. Two Efficient Methods for the Conjugation of Smooth-Form Lipopolysaccharides (LPS) with Probes Bearing Hydrazine or Amino Groups. II. LPS Activation with a Cyanopyridinium Agent. Mold Fungus Toxins
- Extraction and Analysis of Fumonisins and Compounds Indicative of Fumonisin Exposure in Plant and Mammalian Tissues and Cultured Cells. Determination of Fumonisins B1 and B2 in Maize Food Products by a New Analytical Method Based on High-Performance Liquid Chromatography and Fluorometric Detection with Post-Column Derivatization. A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Clean-Up and High-Performance Liquid Chromatography with Fluorometric Detection. Simultanious Determination of Aflatoxins B1, B2, G1 and G2 in Foods and Feed Materials by High-Performance Liquid Chromatography with On-Line Photochemical Derivatization and Fluorescence Detection. Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus.A Rapid Enzymatic Method for Aflatoxin B Detection.