Histology Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Hewitson, Tim D. (Editor ), Darby, Ian A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edición:1st ed. 2010.
Colección:Methods in Molecular Biology, 611
Materias:
Tabla de Contenidos:
  • Tissue Processing
  • Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy
  • An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue
  • Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus
  • Staining Techniques
  • Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells
  • Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems
  • Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections
  • High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy
  • Lectin Histochemistry for Light and Electron Microscopy
  • Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain
  • Special Stains for Extracellular Matrix
  • Active Staining of Mouse Embryos for Magnetic Resonance Microscopy
  • Immunohistochemical Detection of Tumour Hypoxia
  • In Situ Localization of Apoptosis Using TUNEL
  • Imaging Techniques
  • Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord
  • High-Resolution Confocal Imaging in Tissue
  • Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography
  • Image Analysis and Quantitative Morphology.