Protein Gel Detection and Imaging : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Kurien, Biji T. (Editor ), Scofield, R. Hal. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1853
Materias:
Tabla de Contenidos:
  • Protein Stains and Applications
  • The Roles of Acetic Acid and Methanol during Fixing and Staining Proteins in a SDS Polyacrylamide Electrophoresis Gel
  • Multicolored Prestained Standard Protein Marker Generation using a Variety of Remazol Dyes for Easy Visualization of Protein Bands during SDS-PAGE
  • Coomassie-Brilliant Blue Staining of Polyacrylamide Gels
  • A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate Polyacrylamide Gel using Coomassie Blue
  • Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue
  • Silver Staining Techniques of Polyacrylamide Gels
  • Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry
  • Detection of Phosphoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis using 8-Quinolinol Stain
  • Microwave Assisted Protein Staining, De-Staining and In-Gel/In-Solution Digestion of Proteins
  • Fluorescent Staining of Gels
  • A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta during Western Blot Analysis
  • TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE
  • Detection of Glycoproteins in Polyacrylamide Gels using Pro-Q Emerald 300 Dye, A Fluorescent Periodate Schiff-Base Stain
  • Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain
  • Detection of Multiple Enzymes in Fermentation Broth using Single PAGE Analysis
  • Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis
  • A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins
  • Tem Minute stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct red 81 and Amido Black
  • In-Gel Protein Phosphatase Assay using Fluorogenic Substrates
  • Detection of Proteins in Polyacrylamide Gels via Pre-Labeling by Isatoic Anhydride
  • Fluorescent Protein Visualization Immediately after Gel Electrophoresis using an In-Gel Trichloroethanol Photoreaction with Tryptophan
  • Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel
  • Zymographic Determination of Intrinsic Specific Activity of Oxidases in Presence of Interfering Proteins
  • A Simple Method for Detecting Phosphorylation of Proteins by using ZN2+ -Phos-tag SDS-PAGE at Neutral pH
  • Principle and Method of Silver Staining of Proteins Separated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
  • Heat/Pressure Treatment with Detergents Significantly Increases Curcumin Solubility and Stability: Its Use as an Environment-Friendly Protein Gel Stain
  • Fungal Laccase Efficiently Destains Coomassie Brilliant Blue-R-250 Stained Polyacrylamide Gels
  • Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate Polyacrylamide Protein Gels using a Household Detergent
  • Paper Adsorbents Remove Coomassie Blue from Gel Destain and used Gel Stain in an Environment-Friendly Manner
  • Gel Drying Methods
  • Stained Gels can be Stored for Several Months in Non-Sealed Polyethylene Bags
  • Radiolabeling and Analysis of Labeled Proteins.