Plant Immunity : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: McDowell, John M. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 712
Materias:
Tabla de Contenidos:
  • Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression
  • Fragment Complementation and Co-Immunoprecipitation Assays for Understanding R Protein Structure and Function
  • Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
  • Biochemical Purification of Native Immune Protein Complexes
  • Chromatin Immunoprecipitation (ChIP) to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity
  • Dose Response to and Systemic Movement of Dexamethasone in the GVG Inducible Transgene System in Arabidopsis
  • Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis
  • Constructing Haustorium-Specific cDNA Libraries from Rust Fungi
  • Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes
  • Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains
  • A Straightforward Protocol for Electro-Transformation of Phytophthora capsici Zoospores
  • Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis, a Model Oomycete Pathogen of Arabidopsis
  • Assaying Effector Function In planta Using Double-Barreled Particle Bombardment
  • Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast
  • Purification of Effector-Target Protein Complexes via Transient Expression in N. benthamiana
  • Imaging Fluorescently-Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue
  • Immunolocalization of Pathogen Effectors
  • Laser Capture Microdissection of Nematode Feeding Cells
  • Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling
  • Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites
  • Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins.