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03109nam a22003975i 4500 |
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000288074 |
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20210908132851.0 |
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cr nn 008mamaa |
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100805s2010 xxu| s |||| 0|eng d |
| 020 |
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|a 9781607617693
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| 024 |
7 |
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|a 10.1007/978-1-60761-769-3
|2 doi
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| 040 |
|
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a RNAi and microRNA-Mediated Gene Regulation in Stem Cells :
|b Methods, Protocols, and Applications /
|c edited by Baohong Zhang, Edmund J. Stellwag.
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| 250 |
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|a 1st ed. 2010.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2010.
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| 300 |
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|a XI, 240 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 650
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| 505 |
0 |
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|a RNAi in Stem Cells: Current Status and Future Perspectives -- A Brief Introduction to RNAi and MicroRNAs in Stem Cells -- RNA Interference -- Forward RNAi Screens in Human Stem Cells -- A Recessive Genetic Screen for Components of the RNA Interference Pathway in Mouse Embryonic Stem Cells -- Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells -- Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides -- Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector -- Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8 -- Silencing of Rho-GDI? by RNAi Promotes the Differentiation of Neural Stem Cells -- RNAi Knockdown of Redox Signaling Protein Ape1 in the Differentiation of Mouse Embryonic Stem Cells -- Proteins Involved in Cell Migration from Glioblastoma Neurospheres Analyzed by Overexpression and siRNA-Mediated Knock-Down -- An Efficient Transfection Method for Mouse Embryonic Stem Cells -- Semi-quantitative Analysis of Transient Single-Cell Gene Expression in Embryonic Stem Cells by Femtoinjection -- MicroRNAs -- Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Technology -- Assessing In Vivo MicroRNA Function in the Germline Stem Cells of the Drosophila Ovary -- Monitoring MicroRNA Expression During Embryonic Stem-Cell Differentiation Using Quantitative Real-Time PCR (qRT-PCR) -- Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology -- Efficient Gene Knockdowns in Mouse Embryonic Stem Cells Using MicroRNA-Based shRNAs.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
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0 |
|a Stem cells.
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| 650 |
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0 |
|a Gene therapy.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Biochemistry, general.
|
| 650 |
2 |
4 |
|a Human Genetics.
|
| 650 |
2 |
4 |
|a Stem Cells.
|
| 650 |
2 |
4 |
|a Gene Therapy.
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| 700 |
1 |
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|a Zhang, Baohong.
|e editor.
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| 700 |
1 |
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|a Stellwag, Edmund J.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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