Myogenesis : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edición: | 1st ed. 2012. |
| Colección: | Methods in Molecular Biology,
798 |
| Materias: |
Tabla de Contenidos:
- Isolation and Characterization of Human Fetal Myoblasts
- Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Culture System
- Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting (FACS) Cytometry
- Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles
- Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control
- Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary Studies
- Somite Unit Chronometry to Analyze Teratogen Phase Specificity in the Paraxial Mesoderm
- Analysis of Skeletal Muscle Development in Drosophila
- Immunocytochemistry to Study Myogenesis in Zebrafish
- Immunofluorescent Localization of Proteins in C. elegans Muscle
- Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle
- Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading
- Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms Controlling Fiber Size
- In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique
- Recombinant Adeno-Associated Viral Vector Production and Purification
- Generation of Lentiviral Vectors for Use in Skeletal Muscle Research
- Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice
- Gene Profiling Studies in Skeletal Muscle by Quantitative Real-Time Polymerase Chain Reaction Assay
- Analysis of Lipid Profiles in Skeletal Muscles
- Proteomic Analysis of Dystrophic Muscle
- Detection of Calcium Release via Ryanodine Receptors
- Measurement of Calcium Release Due to Inositol Trisphosphate Receptors in Skeletal Muscle
- Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers
- Analysis of Calcium Transients in Cardiac Myocytes and Assessment of the Sarcoplasmic Reticulum Ca2+-ATPase Contribution
- Analysis of Muscle Gene Transcription in Cultured Skeletal Muscle Cells
- Analysis of Fiber Type Differences in Reporter Gene Expression of b-Gal Transgenic Muscle
- Determination of Gene Promoter Activity in Skeletal Muscles In Vivo
- Determination of MiRNA Targets in Skeletal Muscle Cells
- shRNA-Mediated Gene Knockdown in Skeletal Muscle
- Detection of NF-kB Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis
- Isolation of Nuclei from Skeletal Muscle Satellite Cells and Myofibers for Use in Chromatin Immunoprecipitation Assays
- An Improved Restriction Enzyme Accessibility Assay (REAA) for Analyzing Changes in Chromatin Structure in Samples of Limited Cell Number
- ChIP Enriched in Silico Targets (ChEST), a ChIP on Chip Approach Applied to Analyzing Skeletal Muscle Genes.