Notch Signaling : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2014.
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| Edición: | 1st ed. 2014. |
| Colección: | Methods in Molecular Biology,
1187 |
| Materias: |
Tabla de Contenidos:
- Introduction to Notch Signaling
- Genetic Screens to Identify New Notch Pathway Mutants in Drosophila
- Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes
- Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice
- Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster
- Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila
- Tracking Trafficking of Notch and its Ligands in Mammalian Cells
- Visualizing Notch Signaling in vivo in Drosophila Tissues
- Monitoring Notch Activity in the Mouse
- Notch Signaling Assays in Drosophila Cultured Cell Lines
- Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays
- Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays
- Visualization of Notch Signaling Oscillation in Cells and Tissues
- Proteomic Analysis of the Notch Interactome
- Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands
- Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry
- Assay to Probe Proteolytic Processing of Notch by γ-secretase
- Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay
- Identifying Direct Notch Transcriptional Targets using the GSI-washout Assay
- Probing the Epigenetic Status at Notch Target Genes
- Notch-ligand Binding Assays in Drosophila Cells
- Modeling Notch Signaling: A Practical Tutorial
- Small Molecules that Inhibit Notch Signaling
- Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling
- Application of Anti-ligand Antibodies to Inhibit Notch Signaling.