Recombinant and In Vitro RNA Synthesis : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edición: | 1st ed. 2012. |
| Colección: | Methods in Molecular Biology,
941 |
| Materias: |
Tabla de Contenidos:
- Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold.-Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
- Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR
- General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE
- Preparation of Short RNA by in vitro Transcription
- Native RNA Purification by Gel Filtration Chromatography
- Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' Ends
- Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends
- Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods.-Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA
- Affinity Purification of RNA using an ARiBo Tag
- Plasmid Template Design and in vitro Transcription of Short RNAs within a 'Structure Cassette' for Structure Probing Experiments
- In Vitro Transcription of Modified RNAs
- End-Labeling Oligonucleotides with Chemical Tags After Synthesis
- High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA
- Se-derivatized RNAs for X-ray Crystallography
- Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs
- Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC
- Splint Ligation of RNA with T4 DNA Ligase.