NF-kappa B : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: May, Michael J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1280
Materias:
Tabla de Contenidos:
  • Electrophoretic Mobility Shift Assay Analysis of NF-B DNA Binding
  • Detection of IkB Degradation Dynamics and IkB-a Ubiquitination
  • Measurement of NF-κB Transcriptional Activity and Identification of NF-κB cis-regulatory Elements using Luciferase Assays
  • Assessing Sites of NF-κB DNA Binding using Chromatin Immunoprecipitation
  • IKK Kinase Assay for Assessment of Canonical NF-kB Activation in Neurons
  • Analysis of Epidermal Growth Factor-induced NF-kB Signaling
  • Methods to Assess the Activation of the Alternative (non-canonical) NF-kB Pathway by Non-death TNF Receptors
  • Systematic Detection of Non-canonical NF-κB Activation
  • Non-canonical NF-κB activation and SDF-1 expression in Human Endothelial Cells
  • Stable Reconstitution of IKK-deficient Mouse Embryonic Fibroblasts
  • Dissecting NF-κB Signaling Induced by Genotoxic Agents Via Genetic Complementation of NEMO-deficient 1.3E2 Cells
  • Visualizing TCR induced POLKADOTS Formation and NF-κB Activation in the D10 T Cell Clone and Mouse Primary Effector T cells
  • Detection of Recombinant and Cellular MALT1 Paracaspase Activity
  • TRAF Protein Function In Non-Canonical NF-κB Signaling
  • Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-kB Signaling
  • Elucidating Dynamic Protein-protein Interactions and Ubiquitination in NF-kB Signaling Pathways
  • Immunoblot Analysis of Linear Polyubiquitination of NEMO
  • In vitro Detection of NEMO-Ubiquitin Binding using DELFIA and Microscale Thermophoresis Assays
  • Use of Fluorescence Spectroscopy For Quantitative Investigations Of Ubiquitin Interactions with the Ubiquitin-Binding Domains of NEMO
  • Generation of a Proteolytic Signal: E3/E2-mediated Polyubiquitination of IκBα
  • Control of NF-κB Subunits by Ubiquitination
  • Methodology to Study NF-kB/RelA Ubiquitination in vivo
  • Using Sequential Immunoprecipitation and Mass Spectrometry to Identify Methylation of NF-kB
  • Methods to Detect NF-kB Acetylation and Methylation
  • A Method for the Quantitative Analysis of Stimulation-Induced Nuclear Translocation of the p65 subunit of NF-kB from Patient-Derived Dermal Fibroblasts
  • Methods for Assessing the In Vitro Transforming Activity of NF-κB Transcription Factor c-Rel and Related Proteins
  • Using RNA Interference in Lung Cancer Cells to Target the IKK-NF-κB Pathway
  • Immunohistochemical Analysis of NF-kB in Human Tumor Tissue
  • Assessment of Canonical NF-kB Activity in Canine Diffuse Large B-Cell Lymphoma
  • NEMO Binding Domain Peptide Inhibition of Inflammatory Signal-Induced NF-κB Activation In Vivo
  • Regulation of NFκB Signaling in Osteoclasts and Myeloid Progenitors
  • NF-κB Activation with Aging:  Characterization and Therapeutic Inhibition
  • The “Sneaking-ligand” Approach: Cell-type Specific Inhibition of the Classical NF-kB Pathway
  • Sneaking-ligand Fusion Proteins Attenuate Serum Transfer Arthritis by Endothelium-targeted NF-kB inhibition
  • Analysis of NF-kB Activation in Mouse Intestinal Epithelial Cells
  • Characterizing the DNA Binding Site Specificity of NF-kB with Protein Binding Microarrays (PBMs)
  • Methods for Analyzing the Evolutionary Relationship of NF-kB Proteins Using Free, Web-driven Bioinformatics and Phylogenetic Tools
  • Studying NFkB Signaling with Mathematical Models.