RNA Silencing : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Carmichael, Gordon. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2005.
Edición:1st ed. 2005.
Colección:Methods in Molecular Biology, 309
Materias:
Tabla de Contenidos:
  • Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method
  • Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis
  • Preparation and Analysis of Drosha
  • RNA Interference in Caenorhabditis elegans
  • Down-Regulating Gene Expression by RNA Interference in Trypanosoma brucei
  • Analysis of Double-Stranded RNA and Small RNAs Involved in RNA-Mediated Transcriptional Gene Silencing
  • Strategies for the Design of Random siRNA Libraries and the Selection of anti-GFP siRNAs
  • Silencing Gene Expression with Dicer-Generated siRNA Pools
  • Inhibition of Gene Expression In Vivo Using Multiplex siRNA
  • Gene Silencing by a DNA Vector-Based RNAi Technology
  • Plasmid-Based RNA Interference
  • siRNA Delivery In Vivo
  • Peptide-Based Strategy for siRNA Delivery into Mammalian Cells
  • Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells
  • Exon-Specific RNA Interference
  • Immunoprecipitation of MicroRNPs and Directional Cloning of MicroRNAs
  • Detection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In Vitro
  • High-Throughput Analysis of MicroRNA Gene Expression Using Sensitive Probes
  • Modification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNA.